• Before you start
• MutationDB collects mutations of 578 E. coli genome under 178 distinct culture conditions..
• In MutationDB, a condition is described by Strain, Medium, Carbon source, Stress, Generation and other factors.


• Search in the Database
• Click Database on the top navigation bar.
The search service allows a user to search which genome sites are hit by a mutation under a user-defined culture condition and search mutations that hit a gene of interest. The functions offered by the website are annotated as follows:

• Search which genome sites were hit by a mutation under a specific culture condition.
Specifiy a culture condition, by filling in the pulldown menu and then click the search button.
• Search mutations that hit a gene or multiple genes of interest.
Type in the gene(s) of interest in the search box and click search button (A culture condition specification is option).
• Save search result
Click the download button to save the result in a comma separated file.


• Mutation Prediction
Click the Predictor and select a value for each attribute for defining a culture condition, finally click the Predict button. The code for prediction is available at this link.